Knocking at the brain's door: intravital two-photon imaging of autoreactive T cell interactions with CNS structures
2010 | review. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Knocking at the brain's door: intravital two-photon imaging of autoreactive T cell interactions with CNS structures
Kawakami, N.& Fluegel, A. (2010)
Seminars in Immunopathology, 32(3) pp. 275-287.
Springer. DOI: https://doi.org/10.1007/s00281-010-0216-x
Documents & Media
Details
- Authors
- Kawakami, Naoto; Fluegel, Alexander
- Abstract
- Since the first applications of two-photon microscopy in immunology 10 years ago, the number of studies using this advanced technology has increased dramatically. The two-photon microscope allows long-term visualization of cell motility in the living tissue with minimal phototoxicity. Using this technique, we examined brain autoantigen-specific T cell behavior in experimental autoimmune encephalitomyelitis, the animal model of human multiple sclerosis. Even before disease symptoms appear, the autoreactive T cells arrive at their target organ. There they crawl along the intraluminal surface of central nervous system (CNS) blood vessels before they extravasate. In the perivascular environment, the T cells meet phagocytes that present autoantigens. This contact activates the T cells to penetrate deep into the CNS parenchyma, where the infiltrated T cells again can find antigen, be further activated, and produce cytokines, resulting in massive immune cell recruitment and clinical disease.
- Issue Date
- 2010
- Status
- published
- Publisher
- Springer
- Journal
- Seminars in Immunopathology
- ISSN
- 1863-2297