Determination of sugar conformation in large RNA oligonucleotides from analysis of dipole-dipole cross correlated relaxation by solution NMR spectroscopy
1999 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Determination of sugar conformation in large RNA oligonucleotides from analysis of dipole-dipole cross correlated relaxation by solution NMR spectroscopy
Richter, C. ; Griesinger, C. ; Felli, I. C.; Cole, T.; Varani, G. & Schwalbe, H. (1999)
Journal of Biomolecular NMR, 15(3) pp. 241-250. DOI: https://doi.org/10.1023/A:1008319130714
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Details
- Authors
- Richter, C. ; Griesinger, Christian ; Felli, I. C.; Cole, Trevor; Varani, G.; Schwalbe, Harald
- Abstract
- A new experiment, the forward directed quantitative Gamma-HCCH-TOCSY for the measurement of the conformation of the five-membered ribosyl unit in RNA oligonucleotides, is presented. The experiment relies on quantification of cross peak intensities caused by evolution of CH,CH-dipole-dipole cross correlated relaxation in non-evolution periods and the resolution enhancement obtainable in forward directed HCC-TOCSY transfer. Cross correlated relaxation rates are interpreted to reveal the sugar conformation of 22 out of 25 nucleotides in an isotopically labelled 25-mer RNA. The results obtained with this new method are in agreement with the conformational analysis derived from (3)J(H,H) coupling constants.
- Issue Date
- 1999
- Publisher
- Kluwer Academic Publ
- Journal
- Journal of Biomolecular NMR
- ISSN
- 0925-2738
