Transient, phorbol ester-induced DOC2-Munc13 interactions in vivo
1999 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Duncan, Rory R.; Betz, Andrea ; Shipston, Michael J.; Brose, Nils ; Chow, Robert H.
- Abstract
- Munc13-1 and DOC2 have been implicated in the regulation of exocytosis, Here we demonstrate in vivo that these two proteins undergo a transient phorbol ester-mediated and protein kinase C-independent interaction, resulting in the translocation of DOC2 from a vesicular localization to the plasma membrane. The translocation of DOC2 is dependent upon the DOC2 Munc interacting domain that binds specifically to Munc13-1, whereas the association of DOC2 with intracellular membranes is dependent on its C2 domains. This is the first direct in vivo demonstration of a protein-protein interaction between two presynaptic proteins and may represent a molecular basis for phorbol ester-dependent enhancement of exocytosis.
- Issue Date
- 1999
- Journal
- Journal of Biological Chemistry
- ISSN
- 0021-9258
- Language
- English