A new experiment for the measurement of (n)J(C,P) coupling constants including (3)J(C4(i)('),P-i) and (3)J(C4(i)('),Pi+1) in oligonucleotides
1998 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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A new experiment for the measurement of (n)J(C,P) coupling constants including (3)J(C4(i)('),P-i) and (3)J(C4(i)('),Pi+1) in oligonucleotides
Richter, C. ; Reif, B.; Worner, K.; Quant, S.; Marino, J. P.; Engels, J. W. & Griesinger, C. et al. (1998)
Journal of Biomolecular NMR, 12(2) pp. 223-230. DOI: https://doi.org/10.1023/A:1008258226683
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Details
- Authors
- Richter, C. ; Reif, Bernd; Worner, K; Quant, S.; Marino, J. P.; Engels, J. W.; Griesinger, Christian ; Schwalbe, Harald
- Abstract
- A new experiment for the measurement of (n)J(C,P) coupling constants along the phosphodiester backbone in RNA and DNA based on a quantitative-J HCP experiment is presented. In addition to coupling constants, in which a carbon atom couples to only one phosphorus atom, both the intraresidual (3)J(C4(i)',Pi) and the sequential (3)J(C4(i)',Pi+1) for the C4' resonances that couple to two phosphorus atoms can be obtained. Coupling constants obtained by this new method are compared to values obtained from the P-FIDS experiment. Together with (3)J(H,P) coupling constants measured using the P-FIDS experiment, the backbone angles beta and epsilon can be determined.
- Issue Date
- 1998
- Publisher
- Kluwer Academic Publ
- Journal
- Journal of Biomolecular NMR
- ISSN
- 0925-2738
