Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes
1997-02-07 | Zeitschriftenartikel; Forschungsarbeit. Eine Publikation mit Affiliation zur Georg-August-Universität Göttingen.
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Dokumente & Medien
Details
- Autor(en)
- Radler, J. O.; Koltover, I; Salditt, Tim ; Safinya, C. R.
- Zusammenfassung
- Cationic liposomes complexed with DNA (CL-DNA) are promising synthetically based nonviral carriers of DNA vectors for gene therapy. The solution structure of CL-DNA complexes was probed on length scales from subnanometer to micrometer by synchrotron x-ray diffraction and optical microscopy. The addition of either linear lambda-phage or plasmid DNA to CLs resulted in an unexpected topological transition from liposomes to optically birefringent liquid-crystalline condensed globules. X-ray diffraction of the globules revealed a novel multilamellar structure with alternating lipid bilayer and DNA monolayers. The lambda-DNA chains form a one-dimensional lattice with distinct interhelical packing regimes. Remarkably, in the isoelectric point regime, the lambda-DNA interaxial spacing expands between 24.5 and 57.1 angstroms upon lipid dilution and is indicative of a long-range electrostatic-induced repulsion that is possibly enhanced by chain undulations.
- Erscheinungsdatum
- 7-Februar-1997
- Zeitschrift
- Science
- Organisation
- Institut für Röntgenphysik
- Arbeitsgruppe
- RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)
- ISSN
- 0036-8075
- Schlagwort(e)
- membrane biophysics