Planar Cell Movements and Oriented Cell Division During Early Primitive Streak Formation in the Mammalian Embryo
2011 | journal article. A publication with affiliation to the University of Göttingen.
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Planar Cell Movements and Oriented Cell Division During Early Primitive Streak Formation in the Mammalian Embryo
Halacheva, V.; Fuchs, M.; Doenitz, J. ; Reupke, T.; Pueschel, B. & Viebahn, C. (2011)
Developmental Dynamics, 240(8) pp. 1905-1916. DOI: https://doi.org/10.1002/dvdy.22687
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- Authors
- Halacheva, Viktoriya; Fuchs, Mathias; Doenitz, Juergen ; Reupke, Tobias; Pueschel, Bernd; Viebahn, Christoph
- Abstract
- Formation of the mammalian primitive streak appears to rely on cell proliferation to a minor extent only, but compensating cell movements have not yet been directly observed. This study analyses individual cell migration and proliferation simultaneously, using multiphoton and differential interference contrast time-lapse microscopy of late pregastrulation rabbit blastocysts. Epiblast cells in the posterior gastrula extension area accumulated medially and displayed complex planar movements including U-turns and a novel type of processional cell movement. In the same area metaphase plates tended to be aligned parallel to the anterior-posterior axis, and statistical analysis showed that rotations of metaphase plates causing preferred orientation were near-complete 8 min before anaphase onset; in some cases, rotations were strikingly rapid, achieving up to 45 degrees per min. The mammalian primitive streak appears to be formed initially with its typically minimal anteroposterior elongation by a combination of oriented cell divisions with dedicated planar cell movements. Developmental Dynamics 240:1905-1916, 2011. (C) 2011 Wiley-Liss, Inc.
- Issue Date
- 2011
- Status
- published
- Publisher
- Wiley-blackwell
- Journal
- Developmental Dynamics
- ISSN
- 1058-8388
- Sponsor
- Deutsche Forschungsgemeinschaft [Vi 151/8-1]