Fatty acylation of synaptotagmin in PC12 cells and synaptosomes
1996 | journal article; research paper. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Fatty acylation of synaptotagmin in PC12 cells and synaptosomes
Chapman, E. R.; Blasi, J.; An, S.; Brose, N. ; Johnston, P.; Suedhof, T. C. & Jahn, R. (1996)
Biochemical and Biophysical Research Communications, 225(1) pp. 326-332. DOI: https://doi.org/10.1006/bbrc.1996.1174
Documents & Media
Details
- Authors
- Chapman, Edwin R.; Blasi, J.; An, S.; Brose, Nils ; Johnston, PA; Suedhof, Thomas C.; Jahn, Reinhard
- Abstract
- Synaptotagmin I is localized to synaptic vesicles where it functions in the calcium-triggered release of neurotransmitters. Here we demonstrate that synaptotagmin I covalently incorporated [H-13]palmitate after metabolic labeling of PC-12 cells and rat brain synaptosomes. Labeling was localized to a tryptic fragment that contains a cluster of cysteine residues adjacent to the molecule's single transmembrane anchor. Neutral hydroxylamine released the [H-3]palmitate from this fragment and increased its electrophoretic mobility, demonstrating that acylation occurs at the membrane-proximal cysteine cluster. In addition, hydroxylamine-induced mobility shifts were also apparent for synaptotagmins II and III, suggesting that posttranslational palmitoylation via thioester bonds may be a general modification of all synaptotagmins. (C) 1996 Academic Press, Inc.
- Issue Date
- 1996
- Publisher
- Academic Press Inc Jnl-comp Subscriptions
- Journal
- Biochemical and Biophysical Research Communications
- ISSN
- 0006-291X