Balance of pro-apoptotic transforming growth factor-beta and anti-apoptotic insulin effects in the control of cell death in the postnatal mouse retina
2005 | Zeitschriftenartikel. Eine Publikation mit Affiliation zur Georg-August-Universität Göttingen.
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Zitiervorschlag
Balance of pro-apoptotic transforming growth factor-beta and anti-apoptotic insulin effects in the control of cell death in the postnatal mouse retina
Duenker, N.; Valenciano, A. I.; Franke, A.; Hernandez-Sanchez, C.; Dressel, R.; Behrendt, M. & De Pablo, F. u.a. (2005)
European Journal of Neuroscience, 22(1) pp. 28-38. DOI: https://doi.org/10.1111/j.1460-9568.2005.04183.x
Dokumente & Medien
Details
- Autor(en)
- Duenker, N.; Valenciano, A. I.; Franke, A.; Hernandez-Sanchez, C.; Dressel, Ralf; Behrendt, Maik; De Pablo, F.; Krieglstein, Kerstin; de la Rosa, E. J.
- Zusammenfassung
- Transforming growth factor (TGF)-beta and insulin display opposite effects in regulating programmed cell death during vertebrate retina development; the former induces apoptosis while the latter prevents it. In the present study we investigated coordinated actions of TGF-beta and insulin in an organotypic culture system of early postnatal mouse retina. Addition of exogenous TGF-beta resulted in a significant increase in cell death whereas exogenous insulin attenuated apoptosis and was capable of blocking TGF-beta-induced apoptosis. This effect appeared to be modulated via insulin-induced transcriptional down-regulation of TGF-beta receptor II levels. The analysis of downstream signalling molecules also revealed opposite effects of both factors; insulin provided survival signalling by increasing the level of anti-apoptotic Bcl-2 protein expression and phosphorylation and down-regulating caspase 3 activity whereas pro-apoptotic TGF-beta signalling reduced Bcl-2 mRNA levels and Bcl-2 phosphorylation and induced the expression of TGF-induced immediate-early gene (TIEG), a Kruppel-like zinc-finger transcription factor, mimicking TGF-beta activity.
- Erscheinungsdatum
- 2005
- Status
- published
- Herausgeber
- Blackwell Publishing
- Zeitschrift
- European Journal of Neuroscience
- ISSN
- 0953-816X