Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate
2015 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate
Schotten, S.; Meijer, M.; Walter, A. M.; Huson, V.; Mamer, L.; Kalogreades, L. & Veer, M. ter et al. (2015)
eLife, 4 art. e05531. DOI: https://doi.org/10.7554/eLife.05531
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Details
- Authors
- Schotten, Sebastiaan; Meijer, Marieke; Walter, Alexander Matthias; Huson, Vincent; Mamer, Lauren; Kalogreades, Lawrence; Veer, Mirelle ter; Ruiter, Marvin; Brose, Nils ; Rosenmund, Christian; Sørensen, Jakob Balslev; Verhage, Matthijs; Cornelisse, Lennart Niels
- Abstract
- The energy required to fuse synaptic vesicles with the plasma membrane ('activation energy') is considered a major determinant in synaptic efficacy. From reaction rate theory, we predict that a class of modulations exists, which utilize linear modulation of the energy barrier for fusion to achieve supralinear effects on the fusion rate. To test this prediction experimentally, we developed a method to assess the number of releasable vesicles, rate constants for vesicle priming, unpriming, and fusion, and the activation energy for fusion by fitting a vesicle state model to synaptic responses induced by hypertonic solutions. We show that complexinI/II deficiency or phorbol ester stimulation indeed affects responses to hypertonic solution in a supralinear manner. An additive vs multiplicative relationship between activation energy and fusion rate provides a novel explanation for previously observed non-linear effects of genetic/pharmacological perturbations on synaptic transmission and a novel interpretation of the cooperative nature of Ca2+-dependent release.
- Issue Date
- 2015
- Journal
- eLife
- ISSN
- 2050-084X
- Language
- English