Phosphorylation-regulated axonal dependent transport of syntaxin 1 is mediated by a Kinesin-1 adapter
2012 | journal article. A publication with affiliation to the University of Göttingen.
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Phosphorylation-regulated axonal dependent transport of syntaxin 1 is mediated by a Kinesin-1 adapter
Chua, J. J. E.; Butkevich, E.; Worseck, J. M.; Kittelmann, M.; Gronborg, M.; Behrmann, E. & Stelzl, U. et al. (2012)
Proceedings of the National Academy of Sciences of the United States of America, 109(15) pp. 5862-5867. DOI: https://doi.org/10.1073/pnas.1113819109
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Details
- Authors
- Chua, John Jia En; Butkevich, Eugenia; Worseck, Josephine M.; Kittelmann, Maike; Gronborg, Mads; Behrmann, Elmar; Stelzl, Ulrich; Pavlos, Nathan J.; Lalowski, Maciej M.; Eimer, Stefan; Wanker, Erich E.; Klopfenstein, Dieter Robert ; Jahn, Reinhard
- Abstract
- Presynaptic nerve terminals are formed from preassembled vesicles that are delivered to the prospective synapse by kinesin-mediated axonal transport. However, precisely how the various cargoes are linked to the motor proteins remains unclear. Here, we report a transport complex linking syntaxin 1a (Stx) and Munc18, two proteins functioning in synaptic vesicle exocytosis at the presynaptic plasma membrane, to the motor protein Kinesin-1 via the kinesin adaptor FEZ1. Mutation of the FEZ1 ortholog UNC-76 in Caenorhabditis elegans causes defects in the axonal transport of Stx. We also show that binding of FEZ1 to Kinesin-1 and Munc18 is regulated by phosphorylation, with a conserved site (serine 58) being essential for binding. When expressed in C. elegans, wild-type but not phosphorylation-deficient FEZ1 (S58A) restored axonal transport of Stx. We conclude that FEZ1 operates as a kinesin adaptor for the transport of Stx, with cargo loading and unloading being regulated by protein kinases.
- Issue Date
- 2012
- Status
- published
- Publisher
- Natl Acad Sciences
- Journal
- Proceedings of the National Academy of Sciences of the United States of America
- ISSN
- 0027-8424