Lung preservation in experimental ischemia/reperfusion injury and lung transplantation: A comparison of natural and synthetic surfactants
2012 | journal article. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Lung preservation in experimental ischemia/reperfusion injury and lung transplantation: A comparison of natural and synthetic surfactants
Knudsen, L.; Boxler, L.; Muehlfeld, C.; Schaefer, I.-M.; Becker, L.; Bussinger, C. & von Stietencron, I. et al. (2012)
The Journal of Heart and Lung Transplantation, 31(1) pp. 85-93. DOI: https://doi.org/10.1016/j.healun.2011.10.002
Documents & Media
Details
- Authors
- Knudsen, Lars; Boxler, Laura; Muehlfeld, Christian; Schaefer, Inga-Marie; Becker, Laura; Bussinger, Christine; von Stietencron, Immanuel; Madershahian, Navid; Richter, Joachim; Wahlers, Thorsten; Wittwer, Thorsten; Ochs, Matthias
- Abstract
- BACKGROUND: Surfactant inactivation results from ischemia/reperfusion injury and plays a major role in the pathogenesis of primary graft dysfunction after clinical lung transplantation. Thus, prophylactic administration of exogenous surfactant preparations before the onset of ischemia/reperfusion has proven to be effective in preserving pulmonary structure and function. Various natural and synthetic surfactant preparations exhibit differences regarding the biochemical composition and biophysical properties. METHODS: In this study we compared the efficacy of preservation of pulmonary structure and function of the natural surfactant preparations Curosurf and Survanta to that of a synthetic surfactant containing an analog of surfactant protein C (SPC-33) in a rat model of ischemia/reperfusion injury. The oxygenation capacity and peak inspiratory pressure during the reperfusion period were recorded. By applying design-based stereology at the light- and electron-microscopic level, pathologic alterations, including alveolar edema, injury of the blood air barrier and the intra-alveolar as well as intracellular surfactant pools, were quantified. RESULTS: The best oxygenation and preservation of lung structure was achieved with Curosuri. Survanta treatment was associated with the most severe injury of the blood air barrier, and SPC-33 demonstrated signs of microatelectasis. The intra-alveolar surfactant pool after Curosurf and SPC-33 was dominated by active surfactant subtypes, whereas Survanta was associated with the highest fraction of inactive surfactant. The intracellular surfactant pool did not show any differences between the treatment groups. CONCLUSIONS: Taken together, Curosurf achieved the best structural and functional lung preservation, whereas Survanta was inferior to both Curosurf and SPC-33. J Heart Lung Transplant 2012;31:85-93 (C) 2012 International Society for Heart and Lung Transplantation. All rights reserved.
- Issue Date
- 2012
- Status
- published
- Publisher
- Elsevier Science Inc
- Journal
- The Journal of Heart and Lung Transplantation
- ISSN
- 1053-2498
- Sponsor
- Deutsche Forschungsgemeinschaft DFG