Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates
2013 | journal article. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates
Indorf, C.; Bode, S.; Boeckx, P.; Dyckmans, J.; Meyer, A.; Fischer, K. & Joergensen, R. G. (2013)
Analytical Letters, 46(14) pp. 2145-2164. DOI: https://doi.org/10.1080/00032719.2013.796558
Documents & Media
Details
- Authors
- Indorf, Caroline; Bode, Samuel; Boeckx, Pascal; Dyckmans, Jens; Meyer, Axel; Fischer, Klaus; Joergensen, Rainer Georg
- Abstract
- A study on the suitability of chromatographic techniques such as high performance anion exchange chromatography (HPAEC) with fluorescence detection (FL) and pulsed amperometric detection (PAD) and reversed phase (RP) chromatography for the determination of galactosamine, glucosamine, mannosamine, and muramic acid in soil hydrolysates was carried out. The reversed phase fluorescence method was rapid, provided good validation parameters, and employed relatively inexpensive instrumentation. The HPAEC methods had slightly higher limits of quantification, 0.6-5.0 mu molL(-1) (HPAEC-FL) and 1.0-10.0 mu molL(-1) (HPAEC-PAD), compared to the reversed phase fluorescence method (0.5-5.0 mu molL(-1)). Various sample pretreatment methods and chromatographic methods were investigated and the advantages and disadvantages of the HPLC methods are discussed.
- Issue Date
- 2013
- Status
- published
- Publisher
- Taylor & Francis Inc
- Journal
- Analytical Letters
- ISSN
- 0003-2719
- Sponsor
- German Research Foundation (DFG)
