Far-Field Autofluorescence Nanoscopy
2010 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Bierwagen, Jakob ; Testa, Ilaria ; Fölling, Jonas ; Wenzel, Dirk ; Jakobs, Stefan ; Eggeling, Christian ; Hell, Stefan W.
- Abstract
- We demonstrate far-field optical imaging at the nanoscale with unlabeled samples Subdiffraaion resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on off cycle to yield an image, a condition fulfilled by various biomolecules The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts
- Issue Date
- 2010
- Journal
- Nano Letters
- ISSN
- 1530-6984
- Language
- English