Cell-intrinsic and -extrinsic control of Treg-cell homeostasis and function revealed by induced CD28 deletion
2013 | journal article. A publication with affiliation to the University of Göttingen.
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Cell-intrinsic and -extrinsic control of Treg-cell homeostasis and function revealed by induced CD28 deletion
Gogishvili, T.; Luehder, F.; Goebbels, S.; Beer-Hammer, S.; Pfeffer, K. & Huenig, T. (2013)
European Journal of Immunology, 43(1) pp. 188-193. DOI: https://doi.org/10.1002/eji.201242824
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- Authors
- Gogishvili, Tea; Luehder, Fred; Goebbels, Sandra; Beer-Hammer, Sandra; Pfeffer, Klaus; Huenig, Thomas
- Abstract
- While the requirement for CD28 and its ligands for the generation and function of natural (n)Treg cells is well established, it has not been possible yet to investigate cell-intrinsic effects after interrupted CD28 expression. Here, we demonstrate a selective loss of Treg cells after disruption of the CD28 gene. The decline in Treg-cell number was accompanied by reduced homeostatic proliferation, probably due to lack of costimulation during self-antigen recognition, and by impaired Treg-cell function including downregulation of CTLA-4. The decline in Treg-cell number was unaffected by thymectomy or by the presence of CD28 expressing T cells within the same animal, indicating that impairment of peripheral homeostasis and function of nTreg cells by CD28 deletion is cell-intrinsic. In contrast, downregulation of CD25, the a chain of the IL-2R, did not occur in the presence of WT T cells, indicating that its expression does not depend on CD28 signals in cis.
- Issue Date
- 2013
- Status
- published
- Publisher
- Wiley-blackwell
- Journal
- European Journal of Immunology
- ISSN
- 1521-4141; 0014-2980