NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins
2014 | journal article. A publication with affiliation to the University of Göttingen.
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NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins
Yao, X.; Duerr, U. H. N.; Gattin, Z.; Laukat, Y.; Narayanan, R. L.; Brueckner, A.-K. & Meisinger, C. et al. (2014)
PLoS ONE, 9(11) art. e112374. DOI: https://doi.org/10.1371/journal.pone.0112374
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Details
- Authors
- Yao, Xuejun; Duerr, Ulrich H. N.; Gattin, Zrinka; Laukat, Yvonne; Narayanan, Rhagavendran L.; Brueckner, Ann-Kathrin; Meisinger, Chris; Lange, Adam; Becker, Stefan; Zweckstetter, Markus
- Abstract
- Membrane proteins play key roles in biology. Determination of their structure in a membrane environment, however, is highly challenging. To address this challenge, we developed an approach that couples hydrogen/deuterium exchange of membrane proteins to rapid unfolding and detection by solution-state NMR spectroscopy. We show that the method allows analysis of the solvent protection of single residues in liposome-embedded proteins such as the 349-residue Tom40, the major protein translocation pore in the outer mitochondrial membrane, which has resisted structural analysis for many years.
- Issue Date
- 2014
- Status
- published
- Publisher
- Public Library Science
- Journal
- PLoS ONE
- ISSN
- 1932-6203