Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex
2014 | journal article. A publication with affiliation to the University of Göttingen.
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Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex
Wysoczanki, P.; Schneider, C.; Xiang, S.; Munari, F.; Trowitzsch, S.; Wahl, M. C. & Luehrmann, R. et al. (2014)
Nature Structural & Molecular Biology, 21(10) pp. 911-918. DOI: https://doi.org/10.1038/nsmb.2889
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Details
- Authors
- Wysoczanki, Piotr; Schneider, Cornelius; Xiang, ShengQi; Munari, Francesca; Trowitzsch, Simon; Wahl, Markus C.; Luehrmann, Reinhard; Becker, Stefan; Zweckstetter, Markus
- Abstract
- The precursor mRNA (pre-mRNA) retention and splicing (RES) complex is a spliceosomal complex that is present in yeast and humans and is important for RNA splicing and retention of unspliced pre-mRNA. Here, we present the solution NMR structure of the RES core complex from Saccharomyces cerevisiae. Complex formation leads to an intricate folding of three components-Snu17p, Bud13p and Pml1p-that stabilizes the RNA-recognition motif (RRM) fold of Snu17p and increases binding affinity in tertiary interactions between the components by more than 100-fold compared to that in binary interactions. RES interacts with pre-mRNA within the spliceosome, and through the assembly of the RES core complex RNA binding efficiency is increased. The three-dimensional structure of the RES core complex highlights the importance of cooperative folding and binding in the functional organization of the spliceosome.
- Issue Date
- 2014
- Status
- published
- Publisher
- Nature Publishing Group
- Journal
- Nature Structural & Molecular Biology
- ISSN
- 1545-9985; 1545-9993