Limited role of free TDP-43 as a diagnostic tool in neurodegenerative diseases
2014 | journal article. A publication with affiliation to the University of Göttingen.
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Limited role of free TDP-43 as a diagnostic tool in neurodegenerative diseases
Feneberg, E.; Steinacker, P.; Lehnert, S.; Schneider, A.; Walther, P.; Thal, D. R. & Linsenmeier, M. et al. (2014)
Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, 15(5-6) pp. 351-356. DOI: https://doi.org/10.3109/21678421.2014.905606
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Details
- Authors
- Feneberg, Emily; Steinacker, Petra; Lehnert, Stefan; Schneider, Anja; Walther, Paul; Thal, Dietmar Rudolf; Linsenmeier, Miriam; Ludolph, Albert C.; Otto, Markus
- Abstract
- TAR DNA-binding protein 43 (TDP-43) is one of the neuropathological hallmarks in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). It is present in patients' blood and cerebrospinal fluid (CSF); however, the source and clinical relevance of TDP-43 measurements in body fluids is uncertain. We investigated paired CSF and serum samples, blood lymphocytes, brain urea fractions and purified exosomes from CSF for TDP-43 by one-(1D), and two-dimensional (2D) Western immunoblotting (WB) and quantitative mass spectrometry (MRM) in patients with ALS, FTLD and non-neurodegenerative diseases. By means of 2D-WB we were able to demonstrate a similar isoform pattern of TDP-43 in lymphocytes, serum and CSF in contrast to that of brain urea fractions with TDP-43 pathology. We found that the TDP-43 CSF to blood concentration ratio is about 1:200. As a possible brain specific fraction we found TDP-43 in exosome preparations from CSF by immunoblot and MRM. We conclude that TDP-43 in CSF originates mainly from blood. Measurements of TDP-43 in CSF and blood are of minor importance as a diagnostic tool, but may be important for monitoring therapy effects of TDP-43 modifying drugs.
- Issue Date
- 2014
- Status
- published
- Publisher
- Informa Healthcare
- Journal
- Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration
- ISSN
- 2167-9223; 2167-8421