Randomised in situ trial on the effect of milk and CPP-ACP on dental erosion

Abstract

Objectives: This randomised in situ study aimed to analyse the effect of milk (with or without 5 ppm F) and CPP-ACP pastes (with or without 900 ppm F) on dental erosion. Methods: The study was a seven phase (5 days each) crossover design involving 15 participants wearing intraoral appliances with enamel and dentine specimens. Specimens were extraorally eroded (erosive soft drink, 6 x 90 s/day) and brushed (2 x 30 s/day, 2 N) using a non-fluoridated toothpaste (negative control). The test products were milk, milk + 5 ppm F (twice daily, each 100 ml/2 min), CPP-ACP paste, CPP-ACP paste + 900 ppm F (3 min/day) or a SnCl2/AmF/NaF mouthrinse (positive control, 30 s/day), which were applied immediately after erosion with the appliances in the oral cavity. In an additional group, a fluoridated toothpaste was used without any additional test product. Tissue loss was determined profilometrically after 5 days and statistically analysed by linear mixed models methodologies (p < 0.05). Results: Compared with the negative control (non-fluoridated toothpaste only, enamel: 2.2 +/- 1.3 mu m; dentine: 3.8 +/- 2.2 mu m), enamel and dentine loss was significantly reduced by the use of fluoridated toothpaste (enamel: 1.1 +/- 1.0 mu m; dentine: 2.4 +/- 1.7 mu m) and the SnCl2/AmF/NaF mouthrinse (1.5 +/- 1.5 mu m; dentine: 1.8 +/- 1.9 mu m). Conclusions: Milk and CPP-ACP were not effective in reducing enamel and dentine loss significantly, independently of the presence of fluoride. Clinical significance: Enamel and dentine erosion were significantly reduced by the use of a fluoridated toothpaste or a SnCl2/AmF/NaF mouthrinse, but not by milk or CPP-ACP under the conditions of the present study. (C) 2014 Elsevier Ltd. All rights reserved.