Interaction of severe acute respiratory syndrome-associated coronavirus with dendritic cells
2006 | journal article. A publication with affiliation to the University of Göttingen.
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Interaction of severe acute respiratory syndrome-associated coronavirus with dendritic cells
Spiegel, M.; Schneider, K.; Weber, F.; Weidmann, M. & Hufert, F. T. (2006)
Journal of General Virology, 87 pp. 1953-1960. DOI: https://doi.org/10.1099/vir.0.81624-0
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Details
- Authors
- Spiegel, Martin; Schneider, K.; Weber, F.; Weidmann, Manfred; Hufert, Frank T.
- Abstract
- Severe acute respiratory syndrome (SARS) of humans is caused by a novel coronavirus of zoonotic origin termed SARS-associated coronavirus (SARS-CoV). The virus induces severe injury of lung tissue, as well as lymphopenia. and destruction of the architecture of lymphatic tissue by as-yet-unknown mechanisms. In this study, the interaction of SAIRS-CoV with dendritic cells (DCs), the key regulators of immune responses, was analysed. Monocyte-clerived DCs were infected with SARS-CoV and analysed for viability, surface-marker expression and alpha interferon (IFN-alpha) induction. SAIRS-CoV infection was monitored by quantitative RT-PCIR, immunofluorescence analysis and recovery experiments. SARS-CoV infected both immature and mature DCs, although replication efficiency was low. Immature DiCs were activated by SARS-CoV infection and by UV-inactivated SARS-CoV. Infected DCs were still viable on day 6 post-infection, but major histocompatibility complex class I upregulation was missing, indicating that DC function was impaired. Additionally, SARS-CoV infection induced a delayed activation of IFN-alpha expression. Therefore, it is concluded that SARS-CoV has the ability to circumvent both the innate and the adaptive immune systems.
- Issue Date
- 2006
- Status
- published
- Publisher
- Soc General Microbiology
- Journal
- Journal of General Virology
- ISSN
- 0022-1317