Global transcriptional analysis of Methanosarcina mazei strain Go1 under different nitrogen availabilities
2006 | journal article. A publication with affiliation to the University of Göttingen.
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Global transcriptional analysis of Methanosarcina mazei strain Go1 under different nitrogen availabilities
Veit, K.; Ehlers, C.; Ehrenreich, A.; Salmon, K.; Hovey, R.; Gunsalus, R. P. & Deppenmeier, U. et al. (2006)
Molecular Genetics and Genomics, 276(1) pp. 41-55. DOI: https://doi.org/10.1007/s00438-006-0117-9
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- Authors
- Veit, K.; Ehlers, Claudia; Ehrenreich, Armin; Salmon, K.; Hovey, R.; Gunsalus, Robert P.; Deppenmeier, U.; Schmitz, Ruth A.
- Abstract
- Certain archaeal species can fix molecular nitrogen under nitrogen limiting conditions although little is known about this process at either the genetic or molecular level. To address this on a genome-wide scale, transcriptional analysis was performed on the model methanogen Methanosarcina mazei strain Go1 using DNA-microarrays. The genomic expression patterns for cells grown under nitrogen fixing conditions versus nitrogen sufficiency (10 mM ammonium) revealed that approximately 5% of all genes are differentially expressed. Besides a small set of genes previously known to be up-regulated under nitrogen limitation, 14 additional genes involved in nitrogen metabolism were identified plus 10 genes encoding potential transcriptional regulators, 13 genes involved in carbon metabolism, 3 genes in general stress response, 8 putative transporter genes, and an additional 21 genes with unknown function. Quantitative reverse transcriptase PCR experiments confirmed the differential expression of a subset of these genes. Promoter analysis revealed a palindromic DNA motif centered nearby the transcriptional start point for several genes up-regulated under nitrogen limitation. A bioinformatics study demonstrated the presence of this motif in the up-stream region of 52 genes genome-wide, the majority of which showed nitrogen dependent differential transcription. We therefore hypothesize that this DNA element is involved in nitrogen control in M. mazei where it may act as a binding site for a regulatory protein.
- Issue Date
- 2006
- Journal
- Molecular Genetics and Genomics
- ISSN
- 1617-4615