Identification and embryonic expression of Wnt2, Wnt4, Wnt5 and Wnt9 in the millipede Glomeris marginata (Myriapoda: Diplopoda)

Abstract

The Wnt genes encode secreted glycoprotein ligands that are key players during animal development. Previous studies revealed the presence of 12 classes of Wnt genes in protostomes, although lineage specific losses of Wnt genes are common. So far, the gene expression profile of only two complete sets of arthropod Wnt genes has been studied; these are the Wnt genes of the fly Drosophila melanogaster and the beetle Tribolium castaneum. Insects, however, do not represent good models for the understanding of Wnt gene evolution because several Wnt genes have been lost in the lineage leading to the insects, or within the different orders of insects. Comparative gene expression data from non-insect arthropods are rare and restricted to a subset of Wnt genes. This study aims to fill this gap and describes four newly detected Wnt genes from the millipede Glomeris marginata (Myriapoda: Diplopoda). Together with previous studies, now 11 Glomeris Wnt genes have been isolated and their expression has been studied. The only predicted but hitherto undetected Wnt gene is Wnt10. The new data provide a platform for the comparison of Wnt gene expression patterns in arthropods and reveal conserved as well as diverged aspects of Wnt gene expression in Arthropoda. Prominent expression of Wnt4 in dorsal tissue implies a role in dorsal segmentation and suggests that Wnt4 may be the predicted substitute for the previously reported missing expression of wg/Wnt1 in dorsal tissue. (C) 2014 Elsevier B.V. All rights reserved.