Single-Molecule STED Microscopy with Photostable Organic Fluorophores

2010 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Single-Molecule STED Microscopy with Photostable Organic Fluorophores​
Kasper, R.; Harke, B.; Forthmann, C.; Tinnefeld, P.; Hell, S.   & Sauer, M.​ (2010) 
Small, 6(13) pp. 1379​-1384​.​ DOI: https://doi.org/10.1002/smll.201000203 

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Authors
Kasper, Robert; Harke, Benjamin; Forthmann, Carsten; Tinnefeld, Philip; Hell, Stefan ; Sauer, Markus
Abstract
The combination of stimulated emission depletion (STED) microscopy with fluorophore stabilization through a reducing and oxidizing system (ROXS) enables the repetitive measurements necessary for 3D or dynamic STED imaging and resolution enhancement at the single-molecule level. A lateral resolution of <30 nm is obtained for raw image data recorded from single organic fluorophores immobilized in aqueous buffer.
Issue Date
2010
Journal
Small 
ISSN
1613-6810
Language
English

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