Formation of conjugated Delta(11) Delta(13)-double bonds by Delta(12)-linoleic acid (1,4)-acyl-lipid-desaturase in pomegranate seeds
2002 | journal article. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Formation of conjugated Delta(11) Delta(13)-double bonds by Delta(12)-linoleic acid (1,4)-acyl-lipid-desaturase in pomegranate seeds
Hornung, E.; Pernstich, C. & Feussner, I. (2002)
EUROPEAN JOURNAL OF BIOCHEMISTRY, 269(19) pp. 4852-4859. DOI: https://doi.org/10.1046/j.1432-1033.2002.03184.x
Documents & Media
Details
- Authors
- Hornung, E.; Pernstich, C.; Feussner, Ivo
- Abstract
- For the biosynthesis of punicic acid (18:3(Delta9Z ,11E ,13Z) ) a (11,14)-linoleoyl desaturase activity has been proposed. To isolate this acyl-lipid-desaturase, PCR-based cloning was used. This approach resulted in the isolation of two complete cDNAs. The first isolated full-length cDNA harbors a sequence of 1350 bp encoding a protein of 395 amino acids. The second cDNA was 1415 bp long encoding a protein of 387 amino acids. For functional identification proteins encoded by the cDNAs were expressed in Saccharomyces cerevisiae , and formation of newly formed fatty acids was analyzed by gas chromatography-free induction decay (GC-FID) and GC/MS. The expression of the heterologous enzymes resulted in the first case in a significant amount of linoleic acid and in the second case, after linoleic acid supplementation, in formation of punicic acid. The results presented here identify one cDNA coding for a classical Delta(12)-acyl-lipid-desaturase. The other one codes for a new type of (1,4)-acyl-lipid-desaturase that converts a cis double bond located in the Delta(12)-position of linoleic acid or gamma-linolenic acid, but not in alpha-linolenic acid, into a conjugated cis-trans double bond system.
- Issue Date
- 2002
- Status
- published
- Publisher
- Blackwell Publishing Ltd
- Journal
- EUROPEAN JOURNAL OF BIOCHEMISTRY
- ISSN
- 0014-2956