Phage vB_BsuP-Goe1: the smallest identified lytic phage of Bacillus subtilis
2016 | journal article. A publication with affiliation to the University of Göttingen.
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- Authors
- Willms, Inka Marie; Hertel, Robert
- Abstract
- Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis. The phage forms round plaques with a matt outline on agar plates containing a B. subtilis Delta 6 lawn. Transmission electron microscopy and genomic analyses revealed that phage vB_BsuP-Goe1 belongs to the Podoviridae family as a new member of the Phi29likevirus genus. The phage genome comprises 18 379 bp that encode for one RNA gene and for 24 open reading frames. Phage vB_BsuP-Goe1 clusters with phage B103 and Nf within the Phi29likevirus genus and is currently the smallest member of this genus. Genome comparison with members of the Phi29likevirus genus reveals a conserved gene set coding for essential replication and morphogenesis functions while the genome extremities are more prone to gene and genome organisation variability.
- Issue Date
- 2016
- Status
- published
- Publisher
- Oxford Univ Press
- Journal
- FEMS Microbiology Letters
- ISSN
- 1574-6968; 0378-1097
- Sponsor
- Georg August University of Goettingen