Fluorogenic Probes for Multicolor Imaging in Living Cells
2016 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Lukinavicius, Grazvydas; Reymond, Luc; Umezawa, Keitaro; Sallin, Olivier; D'Este, E. ; Goettfert, Fabian ; Ta, Haisen ; Hell, Stefan ; Urano, Yasuteru; Johnsson, Kai
- Abstract
- Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-pertneable, and compatible with super resolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.
- Issue Date
- 2016
- Publisher
- Amer Chemical Soc
- Journal
- Journal of the American Chemical Society
- ISSN
- 0002-7863
