Protein synthesis inhibiting clindamycin improves outcome in a mouse model of Staphylococcus aureus sepsis compared with the cell wall active ceftriaxone
2002 | journal article. A publication with affiliation to the University of Göttingen.
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Protein synthesis inhibiting clindamycin improves outcome in a mouse model of Staphylococcus aureus sepsis compared with the cell wall active ceftriaxone
Azeh, I.; Gerber, J.; Wellmer, A.; Wellhausen, M.; Koenig, B.; Eiffert, H. & Nau, R. (2002)
Critical Care Medicine, 30(7) pp. 1560-1564. DOI: https://doi.org/10.1097/00003246-200207000-00027
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- Authors
- Azeh, I.; Gerber, Joachim; Wellmer, A.; Wellhausen, M.; Koenig, B.; Eiffert, Helmut; Nau, R.
- Abstract
- Objective: The release of proinflammatory components from bacteria depends on the mode of action of the antibacterial therapy used. We studied whether this influences mortality in experimental sepsis. Design. In a lethal murine model of Staphylococcus aureus sepsis, animals were randomly assigned to receive the protein synthesis inhibitor clindamycin (CLI) or the beta-lactam ceftriaxone (CRO). Setting. Therapy was introduced subcutaneously 5 hrs after intraperitoneal injection of 107 colony forming units of S. aureus American Type Culture Collection 29213 and was continued every 8 hrs for 3 days. Measurements and Results: Survival was higher in mice receiving CLI (29/50 animals [58%]) than in mice receiving CRO (16/50 animals [32%]; p = 015). Mice treated with CRO died earlier than mice receiving CLI (p = .002). Eight hours after the first antibiotic dose, the motor performance of mice receiving CRO had deteriorated more than it did for mice receiving CLI (p = .009). Higher levels of tumor necrosis factor-alpha were measured in serum (p = .027) and peritoneal fluid (p = .001) of CRO-treated mice. In vitro, CLI released smaller amounts of staphylococcal enterotoxin A than CRO. Conclusions: Antibiotic treatment of Gram-positive sepsis with a protein synthesis inhibitor decreases morbidity and mortality compared with a bacteriolytic compound. This may be caused by a reduction of the concentrations of proinflammatory/toxic bacterial components and cytokines.
- Issue Date
- 2002
- Status
- published
- Publisher
- Lippincott Williams & Wilkins
- Journal
- Critical Care Medicine
- ISSN
- 0090-3493