Development of a monoclonal blocking ELISA for the detection of antibodies against fowlpox virus
2002 | journal article. A publication with affiliation to the University of Göttingen.
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Development of a monoclonal blocking ELISA for the detection of antibodies against fowlpox virus
Isa, G.; Pfister, K.; Kaaden, O. R. & Czerny, C.-P. (2002)
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 49(1) pp. 21-23. DOI: https://doi.org/10.1046/j.1439-0450.2002.00533.x
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- Authors
- Isa, G.; Pfister, K.; Kaaden, O. R.; Czerny, Claus-Peter
- Abstract
- To provide a fast and easy method to detect antibodies against fowlpox virus (FWPV) particularly in high numbers of chicken sera we established a monoclonal blocking enzyme-linked immunosorbent assay (ELISA). We chose two different monoclonal antibodies (mAb), anti-FWPV 3D9/2B3 and anti-FWPV 8F3/2E11, which are both directed against the 39-kDa protein of FWPV strain HP-1. The blocking ELISA depends on the blocking of mAb binding to solid-phase antigen in the presence of positive serum. For an epidemiological study a total of 184 serum samples from Gambian chicken flocks were analysed against each of the mAbs. Four of the sera were shown to contain FWPV antibodies. These four sera showed a positive cut-off value of more than 50,6 inhibition exclusively in the test against the mAb anti-FWPV 8F3/2E11. This phenomenon can be explained by the binding of the mAbs to distinct epitopes on the same protein.
- Issue Date
- 2002
- Status
- published
- Publisher
- Blackwell Verlag Gmbh
- Journal
- JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
- ISSN
- 0931-1793