Lateral association and elongation of vimentin intermediate filament proteins: A time-resolved light-scattering study
2016-04-21 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Lateral association and elongation of vimentin intermediate filament proteins: A time-resolved light-scattering study
Lopez, C. G.; Saldanha, O.; Huber, K. & Köster, S. (2016)
Proceedings of the National Academy of Sciences, 113(40) pp. 11152-11157. DOI: https://doi.org/10.1073/pnas.1606372113
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Details
- Authors
- Lopez, Carlos G.; Saldanha, Oliva; Huber, Klaus; Köster, Sarah
- Abstract
- Vimentin intermediate filaments (IFs) are part of a family of proteins that constitute one of the three filament systems in the cytoskeleton, a major contributor to cell mechanics. One property that distinguishes IFs from the other cytoskeletal filament types, actin filaments and microtubules, is their highly hierarchical assembly pathway, where a lateral association step is followed by elongation. Here we present an innovative technique to follow the elongation reaction in solution and in situ by time-resolved static and dynamic light scattering, thereby precisely capturing the relevant time and length scales of seconds to minutes and 60-600 nm, respectively. We apply a quantitative model to our data and succeed in consistently describing the entire set of data, including particle mass, radius of gyration, and hydrodynamic radius during longitudinal association.
- Issue Date
- 21-April-2016
- Journal
- Proceedings of the National Academy of Sciences
- Organization
- Institut für Röntgenphysik
- Working Group
- RG Köster (Cellular Biophysics)
- ISSN
- 0027-8424
- Language
- English
- Subject(s)
- cytoskeleton; molecular biophysics