Use of stable isotopes (C-13) for studying the mobilisation of old soil organic carbon by endogeic earthworms (Lumbricidae)

Abstract

Endogeic earthworms ingest large amounts of organic matter enclosed in mineral soil. Part of the soil organic matter is mobilised during the gut passage, but the overall effect of earthworms on the dynamics of soil organic carbon (SOC) is poorly understood because the origin and age of the mobilised SOC pool are unknown. To determine whether endogeic earthworms mobilise old SOC pools, we studied the effect of Octolasion tyrtaeum (Savigny) on C-13 signatures Of CO2 evolved from soil of a maize field incubated in microcosms with and without earthworms for 150 days. Cultivation on this field had changed from wheat (C-3 plant) to maize (C-4 plant) 23 years ago. Earthworms generally decreased in body mass during the experiment but increased CO2 production. Compared to the initial signature (-22.8 +/- 0.1 parts per thousand (PDB)), delta C-13 signatures of O. tyrtaeum were more depleted in the wheat (-23.6 +/- 0.37 parts per thousand(PDB)) and more enriched in the maize soil (-21.0 +/- 0.4 parts per thousand(PDB)) at the end of the experiment. The delta(CO2)-C-13-C signatures in the wheat soil were not affected by earthworms, but earthworms decreased delta(CO2)-C-13-C values in the maize soil during the second half of the incubation period. This indicates enhanced mineralization of old wheat-derived carbon. The results suggest that lumbricid endogeic earthworms contribute to the mobilisation of old carbon pools in soils. (c) 2007 Elsevier Masson SAS. All rights reserved.