The Saccharomyces homolog of mammalian RACK1, Cpc2/Asc1p, is required for FLO11-dependent adhesive growth and dimorphism
2007 | journal article. A publication with affiliation to the University of Göttingen.
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The Saccharomyces homolog of mammalian RACK1, Cpc2/Asc1p, is required for FLO11-dependent adhesive growth and dimorphism
Valerius, O.; Kleinschmidt, M.; Rachfall, N.; Schulze, F.; Marin, S. L.; Hoppert, M. & Streckfuss-Boemeke, K. et al. (2007)
Molecular & Cellular Proteomics, 6(11) pp. 1968-1979. DOI: https://doi.org/10.1074/mcp.M700184-MCP200
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Details
- Authors
- Valerius, Oliver; Kleinschmidt, Malte; Rachfall, Nicole; Schulze, Florian; Marin, Sarai Lopez; Hoppert, Michael; Streckfuss-Boemeke, Katrin; Fischer, Claudia; Braus, Gerhard H.
- Abstract
- Nutrient starvation results in the interaction of Saccharomyces cerevisiae cells with each other and with surfaces. Adhesive growth requires the expression of the FLO11 gene regulated by the Ras/cAMP/cAMP-dependent protein kinase, the Kss1p/MAPK, and the Gcn4p/general amino acid control pathway, respectively. Proteomics two-dimensional DIGE experiments revealed post-transcriptionally regulated proteins in response to amino acid starvation including the ribosomal protein Cpc2p/Asc1p. This putative translational regulator is highly conserved throughout the eukaryotic kingdom and orthologous to mammalian RACK1. Deletion of CPC2/ASC1 abolished amino acid starvation-induced adhesive growth and impaired basal expression of FLO11 and its activation upon starvation in haploid cells. In addition, the diploid Flo11p-dependent pseudohyphal growth during nitrogen limitation was CPC2/ASC1-dependent. A more detailed analysis revealed that a CPC2/ASC1 deletion caused increased sensitivity to cell wall drugs suggesting that the gene is required for general cell wall integrity. Phosphoproteome and Western hybridization data indicate that Cpc2p/Asc1p affected the phosphorylation of the translational initiation factors eIF2 alpha and eIF4A and the ribosome-associated complex RAC. A crucial role of Cpc2p/Asc1p at the ribosomal interface coordinating signal transduction, translation initiation, and transcription factor formation was corroborated.
- Issue Date
- 2007
- Status
- published
- Publisher
- Amer Soc Biochemistry Molecular Biology Inc
- Journal
- Molecular & Cellular Proteomics
- ISSN
- 1535-9484; 1535-9476