The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections
2007 | journal article. A publication with affiliation to the University of Göttingen.
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The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections
Day, J. G.; Lorenz, M.; Wilding, T. A.; Friedl, T.; Harding, K.; Prochold, T. & Brennan, D. et al. (2007)
CRYOLETTERS, 28(5) pp. 359-376.
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Details
- Authors
- Day, John G.; Lorenz, Maike; Wilding, Thomas A.; Friedl, Thomas; Harding, Keith; Prochold, Thomas; Brennan, Debra; Mueller, Julia; Santos, Lilia M. A.; Santos, M. Fatima; Osorio, Hugo C.; Amaral, Raquel; Lukesova, Alena; Hrouzek, Pavel; Lukes, Martin; Elster, Josef; Lukavsky, Jaromir; Probert, Ian; Ryan, Matthew J.; Benson, Erica E.
- Abstract
- Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P<0.05) between cooling regimes were observed where Mr Frosty((R)) was more variable (Inter-Quartile Range being 21.5%, versus 13.0% for CRF samples). Viability assessment using fluorescein diacetate gave significantly (P<0.0001) higher survival than growth in agar with median values being 96% and 89%, respectively. On employing encapsulation-dehydration, greater variability between some validators was observed, with six labs observing recovery in 100% of the beads (84-95% of cells surviving) and one lab observing survival in 80% of the treated beads. Bead disruption followed by algal growth in agar was considered the most reliable and accurate method of assessing cell survival for encapsulation-dehydration.
- Issue Date
- 2007
- Status
- published
- Publisher
- Cryo Letters
- Journal
- CRYOLETTERS
- ISSN
- 0143-2044