Re-citrate synthase from Clostridium kluyveri is phylogenetically related to homocitrate synthase and isopropylmalate synthase rather than to Si-citrate synthase
2007 | journal article. A publication with affiliation to the University of Göttingen.
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Re-citrate synthase from Clostridium kluyveri is phylogenetically related to homocitrate synthase and isopropylmalate synthase rather than to Si-citrate synthase
Li, F.; Hagemeier, C. H.; Seedorf, H.; Gottschalk, G. & Thauer, R. K. (2007)
Journal of Bacteriology, 189(11) pp. 4299-4304. DOI: https://doi.org/10.1128/JB.00198-07
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Details
- Authors
- Li, Fuli; Hagemeier, Christoph H.; Seedorf, Henning; Gottschalk, Gerhard; Thauer, Rudolf K.
- Abstract
- The synthesis of citrate from acetyl-coenzyme A and oxaloacetate is catalyzed in most organisms by a Si-citrate synthase, which is Si-face stereospecific with respect to C-2 of oxaloacetate. However, in Clostridium kluyveri and some other strictly anaerobic bacteria, the reaction is catalyzed by a Re-citrate synthase, whose primary structure has remained elusive. We report here that Re-citrate synthase from C. kluyveri is the product of a gene predicted to encode isopropylmalate synthase. C. kluyveri is also shown to contain a gene for Si-citrate synthase, which explains why cell extracts of the organism always exhibit some Si-citrate synthase activity.
- Issue Date
- 2007
- Status
- published
- Publisher
- Amer Soc Microbiology
- Journal
- Journal of Bacteriology
- ISSN
- 1098-5530; 0021-9193