A lipid bound actin meshwork organizes liquid phase separation in model membranes
2014 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Honigmann, Alf; Sadeghi, Sina; Keller, Jan ; Hell, Stefan ; Eggeling, Christian ; Vink, Richard
- Abstract
- The eukaryotic cell membrane is connected to a dense actin rich cortex. We present FCS and STED experiments showing that dense membrane bound actin networks have severe influence on lipid phase separation. A minimal actin cortex was bound to a supported lipid bilayer via biotinylated lipid streptavidin complexes (pinning sites). In general, actin binding to ternary membranes prevented macroscopic liquid-ordered and liquid-disordered domain formation, even at low temperature. Instead, depending on the type of pinning lipid, an actin correlated multi-domain pattern was observed. FCS measurements revealed hindered diffusion of lipids in the presence of an actin network. To explain our experimental findings, a new simulation model is proposed, in which the membrane composition, the membrane curvature, and the actin pinning sites are all coupled. Our results reveal a mechanism how cells may prevent macroscopic demixing of their membrane components, while at the same time regulate the local membrane composition.
- Issue Date
- 2014
- Publisher
- Elife Sciences Publications Ltd
- Journal
- eLife
- Organization
- Fakultät für Physik
- ISSN
- 2050-084X