In situ detection of bacteria in calcified biofilms using FISH and CARD-FISH
2008 | journal article. A publication with affiliation to the University of Göttingen.
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- Authors
- Shiraishi, Fumito ; Zippel, Barbara; Neu, Thomas R.; Arp, Gernot
- Abstract
- Modified protocols of fluorescence in situ hybridization (FISH) and catalyze reporter deposition fluorescence in situ hybridization (CARD-FISH) were developed in order to detect bacteria in situ in calcified stromatolite biofilms. Smooth, well-preserved thin sections of calcified biofilms (similar to 5 mu m thin, vertical sectioning of -1 cm deep) were obtained by cryo-sectioning using the adhesive tape-stabilization technique. A modified hybridization buffer was applied during hybridization to prevent calcite dissolution as well as false binding of oligonucleotide probes to the charged mineral surfaces. Particularly, bright and specific CARD-FISH signals allowed the detection of bacteria in intensively calcified biofilms even at low magnification, which is suitable for investigating millimeter- to centimeter-scale vertical distribution patterns of bacteria. (C) 2008 Elsevier B.V. All Fights reserved.
- Issue Date
- 2008
- Journal
- Journal of Microbiological Methods
- Organization
- Abteilung Geobiologie ; Geowissenschaftliches Zentrum ; Fakultät für Geowissenschaften und Geographie
- ISSN
- 0167-7012
- Sponsor
- German Research Foundation [DFG-FOR 571; AR 335/5]