Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor
2008 | journal article. A publication with affiliation to the University of Göttingen.
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Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor
Wallace, D. J.; Borgloh, S. M. zum A.; Astori, S.; Yang, Y.; Bausen, M.; Kuegler, S. & Palmer, A. E. et al. (2008)
Nature Methods, 5(9) pp. 797-804. DOI: https://doi.org/10.1038/NMETH.1242
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Details
- Authors
- Wallace, Damian J.; Borgloh, Stephan Meyer zum Alten; Astori, Simone; Yang, Y.; Bausen, Melanie; Kuegler, Sebastian; Palmer, Amy E.; Tsien, Roger Y.; Sprengel, Rolf; Kerr, Jason N. D.; Denk, Winfried; Hasan, Mazahir T.
- Abstract
- Measurement of population activity with single-action-potential, single-neuron resolution is pivotal for understanding information representation and processing in the brain and how the brain's responses are altered by experience. Genetically encoded indicators of neuronal activity allow long-term, cell type-specific expression. Fluorescent Ca2+ indicator proteins (FCIPs), a main class of reporters of neural activity, initially suffered, in particular, from an inability to report single action potentials in vivo. Although suboptimal Ca2+-binding dynamics and Ca2+-induced fluorescence changes in FCIPs are important factors, low levels of expression also seem to play a role. Here we report that delivering D3cpv, an improved fluorescent resonance energy transfer-based FCIP, using a recombinant adeno-associated virus results in expression sufficient to detect the Ca2+ transients that accompany single action potentials. In upper-layer cortical neurons, we were able to detect transients associated with single action potentials firing at rates of <1 Hz, with high reliability, from in vivo recordings in living mice.
- Issue Date
- 2008
- Status
- published
- Publisher
- Nature Publishing Group
- Journal
- Nature Methods
- ISSN
- 1548-7091