Two-photon excitation STED microscopy
2009 | journal article; research paper. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Documents & Media
Details
- Authors
- Moneron, Gael ; Hell, Stefan
- Abstract
- We report sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy achieved by merging this technique with stimulated-emission depletion (STED). We demonstrate an easy-to-implement and promising laser combination based on a short-pulse laser source for two-photon excitation and a continuous-wave (CW) laser source for resolution enhancement. Images of fluorescent nanoparticles and the immunostained transcription regulator NF kappa B in mammalian cell nuclei exhibit resolutions of <50 nm and similar to 70 nm in the focal plane, respectively, corresponding to a 4-5.4-fold improvement over the diffraction barrier.
- Issue Date
- 2009
- Journal
- Optics Express
- ISSN
- 1094-4087
- Language
- English