Phosphoinositide and inositolpolyphosphate signalling in defense responses of Arabidopsis thaliana challenged by mechanical wounding
2008 | journal article. A publication with affiliation to the University of Göttingen.
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Phosphoinositide and inositolpolyphosphate signalling in defense responses of Arabidopsis thaliana challenged by mechanical wounding
Mosblech, A.; Koenig, S.; Stenzel, I.; Grzeganek, P.; Feussner, I. & Heilmann, I. (2008)
Molecular Plant, 1(2) pp. 249-261. DOI: https://doi.org/10.1093/mp/ssm028
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- Authors
- Mosblech, Alina; Koenig, Sabine; Stenzel, Irene; Grzeganek, Peter; Feussner, Ivo; Heilmann, Ingo
- Abstract
- Various biochemical signals are implicated in Arabidopsis wound signalling, including jasmonic acid (JA), salicylic acid, auxin, and Ca2+. Here, we report on cross-talk of phytohormones with phosphoinositide signals not previously implicated in plant wound responses. Within 30 min of mechanical wounding of Arabidopsis rosette-leaves, the levels of the lipid-derived soluble inositolpolyphosphate, inositol 1,4,5-trisphosphate (InsP(3)), increased four to five-fold. Concomitantly, the precursor lipids, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 4-phosphate and phosphatidylinositol transiently depleted, followed by re-synthesis after 30-60 min of stimulation. Increased InsP(3) levels with wounding coincided with JA increases over the first hours of stimulation. In dde2-2-mutant plants deficient in JA biosynthesis, no InsP(3) increase was observed upon wounding, indicating that JA was required for InsP(3) formation, and InsP(3) levels increased in wild-type plants challenged with sorbitol, increasing endogenous JA levels. In InsP 5-ptase plants with attenuated phosphoinositide signalling, the induction of wounding-inducible genes was diminished compared with wildtype plants, suggesting a role for phosphoinositide signalling in mediating plant wound responses. The gene-expression patterns suggest that phosphoinositides contribute to both JA-dependent and JA-independent aspects of wound signalling. Weight gain of Plutella xylostella caterpillars feeding on InsP 5-ptase plants was increased compared with that of caterpillars feeding on wild-type plants. The ecophysiological relevance of phosphoinositide signals in plant defense responses to herbivory is discussed in light of recent findings of inositolpolyphosphate involvement in phytohormone-receptor function.
- Issue Date
- 2008
- Status
- published
- Publisher
- Oxford Univ Press
- Journal
- Molecular Plant
- ISSN
- 1674-2052
- Sponsor
- German Research Foundation [He3424/1-3]