Formation of gutingimycin: analytical investigation of trioxacarcin A-mediated alkylation of dsDNA
2008 | journal article. A publication with affiliation to the University of Göttingen.
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Formation of gutingimycin: analytical investigation of trioxacarcin A-mediated alkylation of dsDNA
Fitzner, A.; Frauendorf, H.; Laatsch, H. & Diederichsen, U. (2008)
Analytical and Bioanalytical Chemistry, 390(4) pp. 1139-1147. DOI: https://doi.org/10.1007/s00216-007-1737-6
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Details
- Authors
- Fitzner, Ansgar; Frauendorf, Holm; Laatsch, Hartmut; Diederichsen, Ulf
- Abstract
- Formation and fragmentation of recognition complexes between trioxacarcin A and various DNA sequences were examined by temperature-dependent UV and CD spectroscopy, HPLC analysis, and ESI mass spectrometry with regard to reaction conditions, intermediates, products, mechanism, and sequence specificity. Cleavage of the trioxacarcin-DNA complexes provided the natural product gutingimycin by guanine abstraction. The resulting DNA with an abasic site was further cleaved into a DNA fragment with a furanyl unit at the 3'-end and an oligonucleotide with a phosphorylated 5'-end.
- Issue Date
- 2008
- Status
- published
- Publisher
- Springer
- Journal
- Analytical and Bioanalytical Chemistry
- ISSN
- 1618-2642
