Expression of stem cell factor receptor c-kit in human nontumoral and tumoral hepatic cells
2009 | journal article. A publication with affiliation to the University of Göttingen.
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Expression of stem cell factor receptor c-kit in human nontumoral and tumoral hepatic cells
Mansuroglu, T.; Baumhoer, D.; Dudas, J.; Haller, F.; Cameron, S.; Lorf, T. & Fuezesi, L. et al. (2009)
European Journal of Gastroenterology & Hepatology, 21(10) pp. 1206-1211. DOI: https://doi.org/10.1097/MEG.0b013e328317f4ef
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- Authors
- Mansuroglu, Tuemen; Baumhoer, Daniel; Dudas, Jozsef; Haller, Florian; Cameron, Silke; Lorf, Thomas; Fuezesi, Laszlo; Ramadori, Giuliano
- Abstract
- Introduction The aim of this study was to examine and compare the stem cell factor receptor c-kit expression in hepatocellular carcinoma (HCC) with the corresponding peritumoral tissue. To confirm the immunohistochemical results in the investigated HCC tissues, HCC cell lines were analyzed for c-kit expression. Methods Expression of c-kit (SCF receptor) has been evaluated in 72 HCC and in the corresponding surrounding nontumorous tissue. Additionally, immunohistochemical analysis reverse transcription-polymerase chain reaction was also used to examine the mRNA expression of c-kit protooncogene in tumor homogenates. Furthermore, three HCC cell lines (HUH-7, HepG2, and SK-Hep1) were used for gene-expression analysis of c-kit mRNA. Results C-kit expression was detected immunohistochemically in 70% of HCC with different degrees of intensity. Moreover, c-kit expression could also be found in about 90% of the corresponding peritumoral noncirrhotic as well as in cirrhotic liver tissues. C-kit mRNA was detectable in 83% of HCC and in 75% of the corresponding peritumoral noncirrhotic as well as in 100% of corresponding peritumoral cirrhotic samples. In addition, two of the three HCC cell lines (HUH-7 and SK-Hep1) showed a well detectable PCR-product for c-kit. Conclusion Hepatocytes express the c-kit receptor at different grade of intensity under normal and altered pathological conditions. The presence of c-kit on HCC cell lines supports the assumption that SCF might play a role in the regulation of proliferative activity of tumorous and nontumorous hepatic cells. Eur J Gastroenterol Hepatol 21:1206-1211 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
- Issue Date
- 2009
- Status
- published
- Publisher
- Lippincott Williams & Wilkins
- Journal
- European Journal of Gastroenterology & Hepatology
- ISSN
- 0954-691X