Driving a planar model system into the 3rd dimension: generation and control of curved pore-spanning membrane arrays
2014 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Driving a planar model system into the 3rd dimension: generation and control of curved pore-spanning membrane arrays
Gleisner, M. ; Mey, I. ; Barbot, M. ; Dreker, C. ; Meinecke, M. & Steinem, C. (2014)
Soft Matter, 10(33) pp. 6228-6236. DOI: https://doi.org/10.1039/c4sm00702f
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Details
- Authors
- Gleisner, Martin ; Mey, Ingo ; Barbot, Mariam ; Dreker, Christina ; Meinecke, Michael ; Steinem, Claudia
- Abstract
- The generation of a regular array of micrometre-sized pore-spanning membranes that protrude from the underlying surface as a function of osmotic pressure is reported. Giant unilamellar vesicles are spread onto non-functionalized Si/SiO2 substrates containing a highly ordered array of cavities with pore diameters of 850 nm, an interpore distance of 4 mm and a pore depth of 10 mm. The shape of the resulting pore-spanning membranes is controlled by applying an osmotic pressure difference between the bulk solution and the femtoliter-sized cavity underneath each membrane. By applying Young-Laplace's law assuming moderate lateral membrane tensions, the response of the membranes to the osmotic pressure difference can be theoretically well described. Protruded pore-spanning membranes containing the receptor lipid PIP2 specifically bind the ENTH domain of epsin resulting in an enlargement of the protrusions and disappearance as a result of ENTH-domain induced defects in the membranes. These results are discussed in the context of an ENTH-domain induced reduction of lateral membrane tension and formation of defects as a result of helix insertion of the protein in the bilayer.
- Issue Date
- 2014
- Journal
- Soft Matter
- Organization
- Institut für Zellbiochemie
- ISSN
- 1744-683X
- eISSN
- 1744-6848
- Language
- English