Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary
2016 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary
Fereydouni, B.; Salinas-Riester, G. ; Heistermann, M. ; Dressel, R. ; Lewerich, L.; Drummer, C. & Behr, R. (2016)
Stem Cells International, 2016 art. 2480298. DOI: https://doi.org/10.1155/2016/2480298
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Details
- Authors
- Fereydouni, Bentolhoda; Salinas-Riester, Gabriela ; Heistermann, Michael ; Dressel, Ralf ; Lewerich, Lucia; Drummer, Charis ; Behr, Rüdiger
- Abstract
- We use the common marmoset monkey (Callithrix jacchus) as a preclinical nonhuman primate model to study reproductive and stem cell biology. The neonatal marmoset monkey ovary contains numerous primitive premeiotic germ cells (oogonia) expressing pluripotent stem cell markers including OCT4A (POU5F1). This is a peculiarity compared to neonatal human and rodent ovaries. Here, we aimed at culturing marmoset oogonia from neonatal ovaries. We established a culture system being stable for more than 20 passages and 5 months. Importantly, comparative transcriptome analysis of the cultured cells with neonatal ovary, embryonic stem cells, and fibroblasts revealed a lack of germ cell and pluripotency genes indicating the complete loss of oogonia upon initiation of the culture. From passage 4 onwards, however, the cultured cells produced large spherical, free-floating cells resembling oocyte-like cells (OLCs). OLCs strongly expressed several germ cell genes and may derive from the ovarian surface epithelium. In summary, our novel primate ovarian cell culture initially lacked detectable germ cells but then produced OLCs over a long period of time. This culture system may allow a deeper analysis of early phases of female primate germ cell development and—after significant refinement—possibly also the production of monkey oocytes.
- Issue Date
- 2016
- Journal
- Stem Cells International
- Project
- SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz
SFB 1002 | C05: Bedeutung von zellulären Immunreaktionen für das kardiale Remodeling und die Therapie der Herzinsuffizienz durch Stammzelltransplantation - Working Group
- RG Dressel
- ISSN
- 1687-9678; 1687-966X
- Language
- English