Microfluidic device for chemical and mechanical manipulation of suspended cells
2018 | journal article. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Microfluidic device for chemical and mechanical manipulation of suspended cells
Rezvani, S.; Shi, N.; Squires, T. M. & Schmidt, C. F. (2018)
Journal of Physics D: Applied Physics, 51(4) pp. 045403. DOI: https://doi.org/10.1088/1361-6463/aaa121
Documents & Media
Details
- Authors
- Rezvani, Samaneh; Shi, Nan; Squires, Todd M.; Schmidt, Christoph F.
- Abstract
- Microfluidic devices have proven to be useful and versatile for cell studies. We here report on a method to adapt microfluidic stickers made from UV-curable optical adhesive with inserted permeable hydrogel membrane micro-windows for mechanical studies of suspended cells. The windows were fabricated by optical projection lithography using scanning confocal microscopy. The device allows us to rapidly exchange embedding medium while observing and probing the cells. We characterize the device and demonstrate the function by exposing cultured fibroblasts to varying osmotic conditions. Cells can be shrunk reversibly under osmotic compression.
- Issue Date
- 2018
- Publisher
- IOP Publishing
- Journal
- Journal of Physics D: Applied Physics
- Project
- info:eu-repo/grantAgreement/EC/FP7/340528/EU//CELLMECHANOCONTROL
- Organization
- Fakultät für Physik
- ISSN
- 0022-3727
- eISSN
- 1361-6463
- ISSN
- 1361-6463
- Language
- German
- Sponsor
- Deutsche Forschungsgemeinschaft (DFG)
European Research Council under the European Union’s Seventh Framework Program