X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (Ovis aries) and European red deer (Cervus elaphus)
2005 | journal article. A publication with affiliation to the University of Göttingen.
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X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (Ovis aries) and European red deer (Cervus elaphus)
Pfeiffer, I. & Brenig, B. (2005)
BMC genetics, 6(16) pp. 1-4. DOI: https://doi.org/10.1186/1471-2156-6-16
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Details
- Title Variant(s)
- methodology article
- Authors
- Pfeiffer, I.; Brenig, B.
- Abstract
- Background: Simple and precise methods for sex determination in animals are a pre-requisite for a number of applications in animal production and forensics. However, some of the existing methods depend only on the detection of Y-chromosome specific sequences. Therefore, the abscence of a signal does not necessarily mean that the sample is of female origin, because experimental errors can also lead to negative results. Thus, the detection of Y- and X-chromosome specific sequences is advantageous. Results: A novel method for sex identification in mammals (sheep, Ovis aries and European red deer, Cervus elaphus) is described, using a polymerase chain reaction (PCR) and sequencing of a part of the amelogenin gene. A partial sequence of the amelogenin gene of sheep and red deer was obtained, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. With a specific pair of primers a DNA fragment of different length between the male and female mammal was amplified.
- Issue Date
- 2005
- Journal
- BMC genetics
- ISSN
- 1471-2156
- Language
- English