An Early Chick Embryo Culture Device for Extended Continuous Observation
2017 | book part. A publication with affiliation to the University of Göttingen.
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Details
- Authors
- Sydow, Hans-Georg ; Pieper, Tobias Karl ; Viebahn, Christoph ; Tsikolia, Nikoloz
- Editors
- Sheng, G.
- Abstract
- Appropriate mechanical tension of the vitelline membrane as the culture substrate for the early chick embryo is frequently reported to be required for successful in vitro development. Here we describe a modified device, made of anodized aluminum, for in vitro culture which we used for studies of left-right symmetry breaking with emphasis on morphology and gene expression as readouts. The technique allows for easy, high-throughput tissue handling and provides a suitable tension in a stable and easily reproducible manner proven to be suitable for correct molecular left-right patterning and heart looping after long-term culture.
- Issue Date
- 2017
- Series
- Methods in Molecular Biology
- ISBN
- 978-1-4939-7215-9
978-1-4939-7216-6 - ISSN
- 1064-3745; 1940-6029
- eISSN
- 1940-6029
- Language
- English