Direct observation of the nanoscale dynamics of membrane lipids in a living cell
2009 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Eggeling, Christian ; Ringemann, Christian; Medda, Rebecca ; Schwarzmann, Günter; Sandhoff, Konrad; Polyakova, Svetlana; Belov, Vladimir N. ; Hein, Birka ; von Middendorff, Claas; Schönle, Andreas ; Hell, Stefan
- Abstract
- Cholesterol-mediated lipid interactions are thought to have a functional role in many membrane-associated processes such as signalling events(1-5). Although several experiments indicate their existence, lipid nanodomains ('rafts') remain controversial owing to the lack of suitable detection techniques in living cells(4,6-9). The controversy is reflected in their putative size of 5-200 nm, spanning the range between the extent of a protein complex and the resolution limit of optical microscopy. Here we demonstrate the ability of stimulated emission depletion (STED) far-field fluorescence nanoscopy(10) to detect single diffusing (lipid) molecules in nanosized areas in the plasma membrane of living cells. Tuning of the probed area to spot sizes similar to 70-fold below the diffraction barrier reveals that unlike phosphoglycerolipids, sphingolipids and glycosylphosphatidylinositol-anchored proteins are transiently (similar to 10-20 ms) trapped in cholesterol-mediated molecular complexes dwelling within <20-nm diameter areas. The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells.
- Issue Date
- 2009
- Journal
- Nature
- ISSN
- 0028-0836
- Language
- English