MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex
2020-01-07 | Zeitschriftenartikel; Forschungsarbeit. Eine Publikation mit Affiliation zur Georg-August-Universität Göttingen.
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Dokumente & Medien
Details
- Autor(en)
- Wang, Cong; Richter-Dennerlein, Ricarda ; Pacheu-Grau, David; Liu, Fan; Zhu, Ying; Dennerlein, Sven; Rehling, Peter
- Zusammenfassung
- The mitochondrial genome encodes for thirteen core subunits of the oxidative phosphorylation system. These proteins assemble with imported proteins in a modular manner into stoichiometric enzyme complexes. Assembly factors assist in these biogenesis processes by providing co-factors or stabilizing transient assembly stages. However, how expression of the mitochondrial-encoded subunits is regulated to match the availability of nuclear-encoded subunits is still unresolved. Here, we address the function of MITRAC15/COA1, a protein that participates in complex I biogenesis and complex IV biogenesis. Our analyses of a MITRAC15 knockout mutant reveal that MITRAC15 is required for translation of the mitochondrial-encoded complex I subunit ND2. We find that MITRAC15 is a constituent of a ribosome-nascent chain complex during ND2 translation. Chemical crosslinking analyses demonstrate that binding of the ND2-specific assembly factor ACAD9 to the ND2 polypeptide occurs at the C-terminus and thus downstream of MITRAC15. Our analyses demonstrate that expression of the founder subunit ND2 of complex I undergoes regulation. Moreover, a ribosome-nascent chain complex with MITRAC15 is at the heart of this process.
- Erscheinungsdatum
- 7-Januar-2020
- Zeitschrift
- EMBO Reports
- Project
- EXC 2067: Multiscale Bioimaging
- Arbeitsgruppe
- RG Rehling (Mitochondrial Protein Biogenesis)
- ISSN
- 1469-221X; 1469-3178
- eISSN
- 1469-3178
- Sprache
- Englisch