3D reconstruction of high-resolution STED microscope images
2008 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Punge, Annedore ; Rizzoli, Silvio ; Jahn, Reinhard ; Wildanger, Jan Dominik; Meyer, Lars ; Schönle, Andreas ; Kastrup, Lars ; Hell, Stefan
- Abstract
- Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100 nm with focused light generally required the use of two lenses in a 4Pi configuration or exceptionally bright photochromic fluorophores. Here, we describe a simple technical solution for 3D nanoscopy of fixed samples: biological specimens are fluorescently labeled, embedded in a polymer resin, cut into thin sections, and then imaged via STED microscopy with nanoscale resolution. This approach allows a 3D image reconstruction with a resolution <80 nm in all directions using available state-of-the art STED microscopes.
- Issue Date
- 2008
- Journal
- Microscopy Research and Technique
- ISSN
- 1059-910X
- Language
- English