Calcium Promotes the Formation of Syntaxin 1 Mesoscale Domains through Phosphatidylinositol 4,5-Bisphosphate
2016 | journal article. A publication with affiliation to the University of Göttingen.
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Calcium Promotes the Formation of Syntaxin 1 Mesoscale Domains through Phosphatidylinositol 4,5-Bisphosphate
Milovanovic, D. ; Platen, M. ; Junius, M. ; Diederichsen, U. ; Schaap, I. A. T. ; Honigmann, A. & Jahn, R. et al. (2016)
Journal of Biological Chemistry, 291(15) pp. 7868-7876. DOI: https://doi.org/10.1074/jbc.M116.716225
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Details
- Authors
- Milovanovic, Dragomir ; Platen, Mitja ; Junius, Meike ; Diederichsen, Ulf ; Schaap, Iwan A. T. ; Honigmann, Alf; Jahn, Reinhard ; van den Bogaart, Geert
- Abstract
- Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a minor component of total plasma membrane lipids, but it has a substantial role in the regulation of many cellular functions, including exo- and endocytosis. Recently, it was shown that PI(4,5)P2and syntaxin 1, a SNARE protein that catalyzes regulated exocytosis, form domains in the plasma membrane that constitute recognition sites for vesicle docking. Also, calcium was shown to promote syntaxin 1 clustering in the plasma membrane, but the molecular mechanism was unknown. Here, using a combination of superresolution stimulated emission depletion microscopy, FRET, and atomic force microscopy, we show that Ca(2+)acts as a charge bridge that specifically and reversibly connects multiple syntaxin 1/PI(4,5)P2complexes into larger mesoscale domains. This transient reorganization of the plasma membrane by physiological Ca(2+)concentrations is likely to be important for Ca(2+)-regulated secretion.
- Issue Date
- 2016
- Journal
- Journal of Biological Chemistry
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- eISSN
- 1083-351X; 0021-9258
- Language
- English