Multi-scale X-ray phase-contrast tomography of murine heart tissue
2020-05-01 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Reichardt, Marius; Frohn, Jasper ; Khan, Amara; Alves, Frauke ; Salditt, Tim
- Abstract
- The spatial organization of cardiac muscle tissue exhibits a complex structure on multiple length scales, from the sarcomeric unit to the whole organ. Here we demonstrate a multi-scale three-dimensional imaging (3d) approach with three levels of magnification, based on synchrotron X-ray phase contrast tomography. Whole mouse hearts are scanned in an undulator beam, which is first focused and then broadened by divergence. Regions-of-interest of the hearts are scanned in parallel beam as well as a biopsy by magnified cone beam geometry using a X-ray waveguide optic. Data is analyzed in terms of orientation, anisotropy and the sarcomeric periodicity via a local Fourier transformation.
- Issue Date
- 1-May-2020
- Journal
- Biomedical Optics Express
- Project
- EXC 2067: Multiscale Bioimaging
- Organization
- Institut für Röntgenphysik
- Working Group
- RG Alves (Translationale Molekulare Bildgebung)
RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics) - ISSN
- 2156-7085
- Language
- English
- Subject(s)
- biomedical tomography
- Sponsor
- Open-Access-Publikationsfonds 2020