X-ray structure analysis of free-standing lipid membranes facilitated by micromachined apertures
2008-05-06 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Beerlink, André ; Wilbrandt, P. -J.; Ziegler, E.; Carbone, D.; Metzger, T. H. ; Salditt, Tim
- Abstract
- Silicon and Teflon substrates have been structured by wet etching and a focused ion beam (FIB) to obtain very defined, clean apertures. Planar, free-standing lipid membranes (black lipid membranes (BLM)) with enhanced long-term stability have been prepared on these apertures by the methods of Montal and Muller(1,2) as well as Miller and Rudin.(3) The stability and geometric control enables the use of X-ray analysis of free-standing single bilayers. With the presented setup, simultaneous structural and electrophysiological measurements will become feasible.
- Issue Date
- 6-May-2008
- Journal
- Langmuir
- Organization
- Institut für Röntgenphysik
- Working Group
- RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)
- ISSN
- 0743-7463
- Subject(s)
- neuro biophysics
